Sanger sequencing.

Optimize your laboratory processes or enhance your research with local sanger sequencing (processed in Colombia) of your PCR products.

General characteristics:
  1. General description: Sanger sequencing reaction from PCR products or plasmids Note: One reaction corresponds to one PCR product sequenced with one primer.
  2. Procedure of sequencing Includes PCR cleanup, sequencing reaction purification, and capillary electrophoresis for sequencing.
  3. Instrument blood sequencing SeqStudio (ThermoFisher).
Requirements:
  • PCR product in a concentration range of 50 to 100ng/ul.
  • Sequencing primers at a concentration of 10 pm/ul.
  • Minimum volume of PCR product and sequencing primer per reaction of 12ul.
  • Information on the type of product to be sequenced and ratio of primers to use.
  • Information on GC content from region to region sequence.
Applications
  • Research.
  • Confirmation of sequence variants (specific InDels).
  • Gene sequencing.
  • Others.
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About

We are a molecular biology laboratory with over 14 years of experience, providing support to researchers and diagnostic Labs in the Latin American region.
We are at your service

In this contact section you can send us any questions you may have about our products or services and we will answer them in a timely manner.
Cambiar imagen Cambiar imagen Cambiar imagen Cambiar imagen
Cambiar imagen Cambiar imagen Cambiar imagen Cambiar imagen
We are a molecular biology laboratory with over 14 years of experience, providing support to researchers and diagnostic Labs in the Latin American region.
We are at your service

In this contact section you can send us any questions you may have about our products or services and we will answer them in a timely manner.
Cambiar imagen Cambiar imagen Cambiar imagen Cambiar imagen
Cambiar imagen Cambiar imagen Cambiar imagen Cambiar imagen